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当前位置: 首页 > 产品中心 > Immunostain_staining_kit > Biomatik/Mouse Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) ELISA kit, Cat#EKU02866/96 Tests/EKU02866-96 Tests
商品详细Biomatik/Mouse Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) ELISA kit, Cat#EKU02866/96 Tests/EKU02866-96 Tests
Biomatik/Mouse Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) ELISA kit, Cat#EKU02866/96 Tests/EKU02866-96 Tests
Biomatik/Mouse Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) ELISA kit, Cat#EKU02866/96 Tests/EKU02866-96 Tests
商品编号: EKU02866-96Tests
品牌: Biomatik inc
市场价: ¥15120.00
美元价: 9072.00
产地: 美国(厂家直采)
公司:
产品分类: 免疫组化染色试剂盒
公司分类: Immunostain_staining_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Format

96-WellPlate

SpeciesReactivity

Musmusculus(Mouse)

SampleType

Tissuehomogenates,CelllysatesandotherBIOLOGicalfluids.

DetectionRange

0.312-20ng/mL

Sensitivity

0.125ng/mL

EstimatedTurnaroundTime

7-11businessdays

TargetName

CalciumChannel,VoltageDependent,R-Type,Alpha1ESubunit

TargetSynonyms

BII,CACNL1A6,CACH6,BraincalciumchannelII,Calciumchannel,Ltype,alpha-1polypeptide,isoform6,Voltage-gatedcalciumchannelsubunitalphaCav2.3

Precision

Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,R-Type,Alpha1ESubunitweretested20timesononeplate,respectively.Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,R-Type,Alpha1ESubunitweretestedon3differentplates,8replicatesineachplate.CV(%)=SD/meanX100;Intra-Assay:CV<10%;inter-assay:><>

DetectionMethod

Colorimetric

AssayTime

3hours

Application

AssayType

Double-antibodySandwich

ShippingCondition

Icepacks

Storage

Shortterm:4°C;Longterm:seemanual.

PrecautionofUse

TheStopSolutionisacidic.Donotallowtocontactskinoreyes.

ExpiryDate

8months

UniProtID

Q61290

Specificity

ThisassayhashighsensitivityandexcellentspecificityfordetectionofCalciumChannel,VoltageDependent,R-Type,Alpha1ESubunit

CrossActivity

Nosignificantcross-reactivityorinterferencebetweenCalciumChannel,VoltageDependent,R-Type,Alpha1ESubunitandanalogueswasobserved.

AssayProcedure

1.Prepareallreagents,samplesandstandards;2.Add100µLstandardorsampletoeachwell.Incubate2hoursat37°C;3.Aspirateandadd100µLpreparedDetectionReagentA.Incubate1hourat37°C;4.Aspirateandwash3times;5.Add100µLpreparedDetectionReagentB.Incubate30minutesat37°C;6.Aspirateandwash5times;7.Add90µLSubstrateSolution.Incubate10-20minutesat37°C;8.Add50µLStopSolution.Readat450nmimmediately.

StABIlity

Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.

Restrictions

ForResearchUseOnly.Notforuseinvitrodiagnosticprocedures,druguse,orforadmiNISTrationtohumansoranimals.

QualitySystems

ThekitismanufacturedatISO9001andISO13485certifiedfacilities.

QualityAssurance

BiomatikELISAkitsarenottypicallypre-assembledasfinishedproductsduetolimitedshelflife.Beforeshipping,eachkitisassembledandtestedtoensurethatitmeetsspecificationsbeforeshipping.MinorchangesmayoccurtotheDetectionRange,Sensitivity,andPrecision.AllkitsaretestedtoconfirmthattheyfallwithintheirdefinedInter-andIntra-assaycoefficientofvariation.PleasealwaysrefertothehardcopymanualincludedinThekitbeforeexperiment.

ResearchArea

Signaltransduction
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