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Biomatik/Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA kit, Cat#EKU03474/96 Tests/EKU03474-96 Tests
Biomatik/Human Corticotropin Releasing Hormone Receptor 2 (CRHR2) ELISA kit, Cat#EKU03474/96 Tests/EKU03474-96 Tests
商品编号：
EKU03474-96Tests
品牌：
Biomatik inc
市场价：
¥14700.00
美元价：
8820.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Format
96-Well Plate
Species Reactivity
Homo sapiens (Human)
Sample Type
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.312-20 ng/mL
Sensitivity
0.115 ng/mL
Estimated Turnaround Time
7-11 business days
Target Name
Corticotropin Releasing Hormone Receptor 2
Target Synonyms
CRFR2, CRF2, Corticotropin-releasing factor receptor 2
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 2 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;inter-assay:><>
Detection Method
Colorimetric
Assay Time
3 hours
Application
ELISA
Assay Type
Double-antibody Sandwich
Shipping Condition
Ice packs
Storage
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
Q13324
Specificity
This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone Receptor 2
Cross Activity
No significant cross-reactivity or interference between Corticotropin Releasing Hormone Receptor 2 and analogues was observed.
Assay Procedure
1.Prepare all reagents, samples and standards; 2.Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3.Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4.Aspirate and wash 3 times; 5.Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6.Aspirate and wash 5 times; 7.Add 90 µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8.Add 50 µL Stop Solution. Read at 450 nm immediately.
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For Research Use Only. Not for use in vitro diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Research Area
Endocrinology
品牌介绍
热烈祝贺蚂蚁淘生物成为加拿大 Biomatik 品牌授权代理经销商
2019年8月苏州蚂蚁淘公司与加拿大 Biomatik 达成战略合作协议，Biomatik授权苏州蚂蚁淘生物经销Biomatik品牌全线产品在中国市场的推广和销售。点击查看 《授权证书》 。
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